Xylanases from Bacillus Subtilis Expressed in B. Subtilis
نویسنده
چکیده
Xylanase is an enzyme that catalyzes the hydrolysis of 1,4-beta-D-xylosidic linkages in xylans that are constituents of hemicellulose, a structural component of plant cell walls. Arabinoxylans (also known as pentosans) are highly branched xylans that occur in wheat and rye flour. Several xylanases from fungal and bacterial sources are currently marketed for use in baking (Amfep; Pariza and Johnson, 2001). Xylanases described in this Chemical and Technical Assessment are derived from genetically engineered nonpathogenic and nontoxigenic strains of Bacillus subtilis. The information about these enzymes is provided in a dossier submitted to JECFA by the sponsor, Danisco, Inc. (Danisco, 2003).
منابع مشابه
Cloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis
in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
متن کاملA novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing.
Although several xylanases have been studied, only few xylanases from marine micro-organisms have been reported. We report here a novel halotolerant xylanase from marine bacterium Bacillus subtilis cho40 isolated from Chorao island of mandovi estuary Goa, India. Extracellular xylanase was produced by using agricultural residue such as wheat bran as carbon source under solid-state fermentation (...
متن کاملCrystallization and preliminary X-ray diffraction study of two complexes of a TAXI-type xylanase inhibitor with glycoside hydrolase family 11 xylanases from Aspergillus niger and Bacillus subtilis.
Endo-beta-1,4-xylanases hydrolyze arabinoxylan, a major constituent of cereal cell walls, and are nowadays widely used in biotechnological processes. Purified complexes of family 11 xylanases from Aspergillus niger and Bacillus subtilis with TAXI I, a TAXI-type xylanase inhibitor from Triticum aestivum L., were prepared. In both cases the complex was crystallized using the hanging-drop vapour-d...
متن کاملConstitutive High Level Expression of an Endoxylanase Gene from the Newly Isolated Bacillus subtilis AQ1 in Escherichia coli
A xylanolytic bacterium was isolated from the sediment of an aquarium. Based on the 16S rDNA sequence as well as morphological and biochemical properties the isolate was identified and denoted as Bacillus subtilis (B. subtilis) AQ1 strain. An endoxylanase-encoding gene along with its indigenous promoter was PCR amplified and after cloning expressed in E. coli. In E. coli the recombinant enzyme ...
متن کاملMolecular Detection of Lipase A gene in Putative Bacillus subtilis Strains Isolated from Soil
The present study was undertaken to screen the soil samples collected in Iran for the presence of the Bacillus subtilis lipase A gene. The bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and the polymerase chain reaction (PCR). Only four colonies were identified as putative B. subtilis strains and all contained the lipase A ge...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2004